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cre hgh aav9  (Addgene inc)


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    Addgene inc cre hgh aav9
    Cre Hgh Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+hgh+aav9/pENN%2EAAV%2EhSyn%2ECre%2EhGH+(Plasmid+%23105555)/pmc13032744-100-0-2
    Average 93 stars, based on 17 article reviews
    cre hgh aav9 - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Astrocyte Proximity Protects Synapses From Human Amyloid-Beta Induced Degeneration in a Mouse Ex Vivo Model of Early Alzheimer's Disease.
    Article Snippet: Cre.hGH (AAV9) (Addgene #105555- AAV9, 2.2 1013 GC/ml), pAAV.syn.flex.jGcamp7b.WPRE (AAV1) (Addgene #104493- AAV1, 1.4 1013 GC/ ml), pAAV.syn.flex.gcamp6s.WPRE.SV40 (AAV9) (Addgene #100845- AAV9, 2.4 1013 GC/ml), pAAV.syn.flex.jGcamp8s.WPRE (AAV9) (Addgene #162377- AAV9, 2.7 1013 GC/ ml), pAAV- Ef1a- DIO- GFP (AAV1) (Addgene #50457- AAV1, 2.2 1013 GC/ml), pAAV- Ef1a- DIO- mScarlet (AAV1) (Addgene #131002- AAV1, 2.1 1013 GC/ml) and pAAV- Ef1a- Con/Foff 2.0- BFP (AAV9) (Addgene #137130- AAV8, 2.1 1013 GC/ml).

    Article Title: Astrocyte Proximity Protects Synapses From Human Amyloid‐Beta Induced Degeneration in a Mouse Ex Vivo Model of Early Alzheimer's Disease
    Article Snippet: Cre.hGH (AAV9) (Addgene #105555‐AAV9, 2.2 10 13 GC/ml), pAAV.syn.flex.jGcamp7b.WPRE (AAV1) (Addgene #104493‐AAV1, 1.4 10 13 GC/ml), pAAV.syn.flex.gcamp6s.WPRE.SV40 (AAV9) (Addgene #100845‐AAV9, 2.4 10 13 GC/ml), pAAV.syn.flex.jGcamp8s.WPRE (AAV9) (Addgene #162377‐AAV9, 2.7 10 13 GC/ml), pAAV‐Ef1a‐DIO‐GFP (AAV1) (Addgene #50457‐AAV1, 2.2 10 13 GC/ml), pAAV‐Ef1a‐DIO‐mScarlet (AAV1) (Addgene #131002‐AAV1, 2.1 10 13 GC/ml) and pAAV‐Ef1a‐Con/Foff 2.0‐BFP (AAV9) (Addgene #137130‐AAV8, 2.1 10 13 GC/ml).



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    cre hgh aav9  (Addgene inc)
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    Addgene inc cre hgh aav9
    Cre Hgh Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+hgh+aav9/pENN%2EAAV%2EhSyn%2ECre%2EhGH+(Plasmid+%23105555)/pmc13032744-100-0-2
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    a ) Representative traces from mEPSC recordings in Syngap1 +/+ (blue) and Syngap1 +/- (red) neurons treated with vehicle or SR-1815 (left) . Plot showing m EPSC frequency for the four different conditions ( middle ) and mEPSC amplitudes for the four different conditions ( right ) n = 17 for Syngap1 +/+ : vehicle; n = 16 for Syngap1 +/+ : SR-1815; n = 17 for Syngap1 +/- : vehicle; n = 18 for Syngap1 +/- : SR-1815. Error bars represent SEM. b Syngap1 + /+ and Syngap1 + /- neurons transduced with <t>AAV9</t> vectors expressing Flex-gCAMP8f and Cre (to control labeling). Neurons were treated with DMSO or 1.5 μM SR-1815 for 14 days. Calcium imaging performed on DIV14. Plot showing the spiking frequency (spikes per second) for the four different conditions. For DMSO control 12 fields were imaged for Syngap1 +/+ Syngap1 +/- and for SR-1815 respectively 14 and 17 fields for Syngap1 +/+ Syngap1 +/- were imaged from at least 4 wells per conditions. The total number of segmented neurons were DMSO Syngap1 +/+ , n = 1693; Syngap1 +/- , n = 2047; SR-1815 Syngap1 +/+ , n = 1803 and Syngap1 +/- , n = 1253. Dots with error bar represent mean and SEM, individual neuron value (small dot) and well average (large dot) are plotted, within the violin plot median, 25th and 75th percentile. p -value for main effects and interaction are indicated as n.s.: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, detailed statistics and source data are provided as a Source Data file.
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    a ) Representative traces from mEPSC recordings in Syngap1 +/+ (blue) and Syngap1 +/- (red) neurons treated with vehicle or SR-1815 (left) . Plot showing m EPSC frequency for the four different conditions ( middle ) and mEPSC amplitudes for the four different conditions ( right ) n = 17 for Syngap1 +/+ : vehicle; n = 16 for Syngap1 +/+ : SR-1815; n = 17 for Syngap1 +/- : vehicle; n = 18 for Syngap1 +/- : SR-1815. Error bars represent SEM. b Syngap1 + /+ and Syngap1 + /- neurons transduced with <t>AAV9</t> vectors expressing Flex-gCAMP8f and Cre (to control labeling). Neurons were treated with DMSO or 1.5 μM SR-1815 for 14 days. Calcium imaging performed on DIV14. Plot showing the spiking frequency (spikes per second) for the four different conditions. For DMSO control 12 fields were imaged for Syngap1 +/+ Syngap1 +/- and for SR-1815 respectively 14 and 17 fields for Syngap1 +/+ Syngap1 +/- were imaged from at least 4 wells per conditions. The total number of segmented neurons were DMSO Syngap1 +/+ , n = 1693; Syngap1 +/- , n = 2047; SR-1815 Syngap1 +/+ , n = 1803 and Syngap1 +/- , n = 1253. Dots with error bar represent mean and SEM, individual neuron value (small dot) and well average (large dot) are plotted, within the violin plot median, 25th and 75th percentile. p -value for main effects and interaction are indicated as n.s.: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, detailed statistics and source data are provided as a Source Data file.
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    a ) Representative traces from mEPSC recordings in Syngap1 +/+ (blue) and Syngap1 +/- (red) neurons treated with vehicle or SR-1815 (left) . Plot showing m EPSC frequency for the four different conditions ( middle ) and mEPSC amplitudes for the four different conditions ( right ) n = 17 for Syngap1 +/+ : vehicle; n = 16 for Syngap1 +/+ : SR-1815; n = 17 for Syngap1 +/- : vehicle; n = 18 for Syngap1 +/- : SR-1815. Error bars represent SEM. b Syngap1 + /+ and Syngap1 + /- neurons transduced with <t>AAV9</t> vectors expressing Flex-gCAMP8f and Cre (to control labeling). Neurons were treated with DMSO or 1.5 μM SR-1815 for 14 days. Calcium imaging performed on DIV14. Plot showing the spiking frequency (spikes per second) for the four different conditions. For DMSO control 12 fields were imaged for Syngap1 +/+ Syngap1 +/- and for SR-1815 respectively 14 and 17 fields for Syngap1 +/+ Syngap1 +/- were imaged from at least 4 wells per conditions. The total number of segmented neurons were DMSO Syngap1 +/+ , n = 1693; Syngap1 +/- , n = 2047; SR-1815 Syngap1 +/+ , n = 1803 and Syngap1 +/- , n = 1253. Dots with error bar represent mean and SEM, individual neuron value (small dot) and well average (large dot) are plotted, within the violin plot median, 25th and 75th percentile. p -value for main effects and interaction are indicated as n.s.: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, detailed statistics and source data are provided as a Source Data file.
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    Image Search Results


    a ) Representative traces from mEPSC recordings in Syngap1 +/+ (blue) and Syngap1 +/- (red) neurons treated with vehicle or SR-1815 (left) . Plot showing m EPSC frequency for the four different conditions ( middle ) and mEPSC amplitudes for the four different conditions ( right ) n = 17 for Syngap1 +/+ : vehicle; n = 16 for Syngap1 +/+ : SR-1815; n = 17 for Syngap1 +/- : vehicle; n = 18 for Syngap1 +/- : SR-1815. Error bars represent SEM. b Syngap1 + /+ and Syngap1 + /- neurons transduced with AAV9 vectors expressing Flex-gCAMP8f and Cre (to control labeling). Neurons were treated with DMSO or 1.5 μM SR-1815 for 14 days. Calcium imaging performed on DIV14. Plot showing the spiking frequency (spikes per second) for the four different conditions. For DMSO control 12 fields were imaged for Syngap1 +/+ Syngap1 +/- and for SR-1815 respectively 14 and 17 fields for Syngap1 +/+ Syngap1 +/- were imaged from at least 4 wells per conditions. The total number of segmented neurons were DMSO Syngap1 +/+ , n = 1693; Syngap1 +/- , n = 2047; SR-1815 Syngap1 +/+ , n = 1803 and Syngap1 +/- , n = 1253. Dots with error bar represent mean and SEM, individual neuron value (small dot) and well average (large dot) are plotted, within the violin plot median, 25th and 75th percentile. p -value for main effects and interaction are indicated as n.s.: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, detailed statistics and source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contexts

    doi: 10.1038/s41467-025-65971-x

    Figure Lengend Snippet: a ) Representative traces from mEPSC recordings in Syngap1 +/+ (blue) and Syngap1 +/- (red) neurons treated with vehicle or SR-1815 (left) . Plot showing m EPSC frequency for the four different conditions ( middle ) and mEPSC amplitudes for the four different conditions ( right ) n = 17 for Syngap1 +/+ : vehicle; n = 16 for Syngap1 +/+ : SR-1815; n = 17 for Syngap1 +/- : vehicle; n = 18 for Syngap1 +/- : SR-1815. Error bars represent SEM. b Syngap1 + /+ and Syngap1 + /- neurons transduced with AAV9 vectors expressing Flex-gCAMP8f and Cre (to control labeling). Neurons were treated with DMSO or 1.5 μM SR-1815 for 14 days. Calcium imaging performed on DIV14. Plot showing the spiking frequency (spikes per second) for the four different conditions. For DMSO control 12 fields were imaged for Syngap1 +/+ Syngap1 +/- and for SR-1815 respectively 14 and 17 fields for Syngap1 +/+ Syngap1 +/- were imaged from at least 4 wells per conditions. The total number of segmented neurons were DMSO Syngap1 +/+ , n = 1693; Syngap1 +/- , n = 2047; SR-1815 Syngap1 +/+ , n = 1803 and Syngap1 +/- , n = 1253. Dots with error bar represent mean and SEM, individual neuron value (small dot) and well average (large dot) are plotted, within the violin plot median, 25th and 75th percentile. p -value for main effects and interaction are indicated as n.s.: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, detailed statistics and source data are provided as a Source Data file.

    Article Snippet: Cell suspension was then diluted into Feeding medium consisting of Neurobasal-A (Invitrogen: 10888022), 2% Glutamax-I, and 0.02% Gentamicin, 2% B-27 supplement (Invitrogen: 17504044), 10 μM 5-fluoro-2′-deoxyuridine (FUDR) (Sigma:F0503) to suppress the proliferation of glia, and 1 × 10e4—3x10e4 viral particles/cell of pENN.AAV.hSyn.Cre.WPRE.hGH (AAV9) (Addgene: 105553-AAV9; single-use aliquots) to induce haploinsufficiency (Fig. ).

    Techniques: Transduction, Expressing, Control, Labeling, Imaging